WebbMATERIALS AND METHODS Sample Preparation A549 (ATCC) were cultured with SILAC media (Thermo Scientific) containing 10% dialyzed FBS and one of 8 different lysine … WebbThe super-SILAC spiked peptide samples were further fractionated using the Pierce high pH-reversed-phase peptide fractionation kit (Thermo Fisher Scientific, Bartlett, IL, USA). …

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WebbA549 cells were labeled in light/medium/heavy SILAC medium. Harvested cells were lysed, digested and mixed to a ratio of 1:1:1 and 2:1:10 for analysis. The mixing accuracy has … Webb12 apr. 2024 · thermo常备现货产品 (578) gibco培养基试剂 gibco科研培养基 poros填料、试剂盒 thermo储液袋 oxoid、bd培养基 ... silac用氨基酸--赛可锐--现货2 重 silac 氨基酸标准品[n/a][n/a] breakneck city ps4

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WebbProteins from ctrl cells (Light SILAC labeled) and from TRAP3high cells (Heavy SILAC labeled) were digested to peptides using trypsin and mixed in a 1:1 proportion. ... WebbCross-linked SILAC labeled cell samples were prepared as described previously . Briefly, HeLa cells (ATCC) were seeded at a density of 5 x 10 6 cells into 150 mm culture dishes with 20 mL of SILAC DMEM medium containing either isotopically light L-lysine and L-arginine or isotopically heavy 13 C 6 15 N 2 -L-lysine and 13 C 6 -L-arginine (Silantes) and … WebbThermo Scientific Powdered RPMI Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein... Shop Thermo … breakneck campground portersville pa